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AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)
(Plasmid #165456)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165456 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAVS1-Puro
  • Backbone size w/o insert (bp) 11520
  • Total vector size (bp) 15160
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ZEB2
  • Alt name
    SIP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3641
  • GenBank ID
    NM_014795.3
  • Entrez Gene
    ZEB2 (a.k.a. HSPC082, SIP-1, SIP1, SMADIP1, ZFHX1B)
  • Promoter CAG
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site FseI (not destroyed)
  • 5′ sequencing primer CGGCGCGAACTGGATCCAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A) was a gift from Madeline Lancaster (Addgene plasmid # 165456 ; http://n2t.net/addgene:165456 ; RRID:Addgene_165456)

  • For your References section:

    An early cell shape transition drives evolutionary expansion of the human forebrain. Benito-Kwiecinski S, Giandomenico SL, Sutcliffe M, Riis ES, Freire-Pritchett P, Kelava I, Wunderlich S, Martin U, Wray GA, McDole K, Lancaster MA. Cell. 2021 Apr 15;184(8):2084-2102.e19. doi: 10.1016/j.cell.2021.02.050. Epub 2021 Mar 24. 10.1016/j.cell.2021.02.050 PubMed 33765444
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