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Addgene

pLD037-pCMV-APOBEC-Cas9(D10A)-rXRCC1
(Plasmid #165444)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165444 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMV
  • Backbone manufacturer
    origene
  • Backbone size w/o insert (bp) 3399
  • Total vector size (bp) 10173
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    APOBEC-nCas9-rXRCC1
  • Alt name
    rAPOBEC1-XTEN-Cas9n-rXRCC1-NLS
  • Species
    R. norvegicus (rat), Synthetic; Streptococcus pyogenes
  • Insert Size (bp)
    6774
  • Entrez Gene
    Apobec1 (a.k.a. REPR, apobec-1)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cgcaaatgggcggtaggcgtg
  • 3′ sequencing primer tagaaggcacagtcgagg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLD037-pCMV-APOBEC-Cas9(D10A)-rXRCC1 was a gift from Wei Leong Chew (Addgene plasmid # 165444 ; http://n2t.net/addgene:165444 ; RRID:Addgene_165444)

  • For your References section:

    Programmable C:G to G:C genome editing with CRISPR-Cas9-directed base excision repair proteins. Chen L, Park JE, Paa P, Rajakumar PD, Prekop HT, Chew YT, Manivannan SN, Chew WL. Nat Commun. 2021 Mar 2;12(1):1384. doi: 10.1038/s41467-021-21559-9. 10.1038/s41467-021-21559-9 PubMed 33654077
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