pAAV-CMV-mTurquoise2-NLS
(Plasmid #165443)

• Purpose: Expresses nuclear mTurquoise2 from CMV promoter
• Depositing Lab: Esteban Engel
• Publication: Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41434-021-00272-8. doi: 10.1038/s41434-021-00272-8.

Want a viral vector made from this plasmid?

Make a packaging request and we'll get back to you.

This service is available to academics and nonprofits only.

Please log in to submit a packaging request.

• Serotype: Select serotype for details See details about
• Pricing: Select serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
• How this works:
  • Place a request for a quantity of 10 (1 mL), 25 (2.5 mL), or 50 (5 mL). Our all-inclusive pricing includes DNA production and QC.
  • Addgene will quickly confirm that we can produce a high-quality prep for you.
  • Track your request and place an order from within your account. Payment information must be added before we can begin processing your order.
  • Receive your prep in 6–9 weeks after the MTA is approved by your organization.
  • Learn more about our Packaged on Request Service.

Backbone

• Vector backbone: pAAV-CMV-EGFP-NLS
• Backbone manufacturer: Esteban Engel lab
• Total vector size (bp): 5130
• Modifications to backbone: Replaced EGFP with mTurquoise2
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mTurquoise2-NLS
• Insert Size (bp): 813
• Promoter: CMV
• Tag / Fusion Protein:
  • NLS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: ataGCTAGCATGGTGAGCAAGGGCGAGG
• 3′ sequencing primer: gttCTCGAGaCTTGTACAGCTCGTCCATGCC

Resource Information

• Supplemental Documents:
  • pAAV-CMV-mTurquoise2-NLS.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry