pBI-p53 R175H/EGFP
(Plasmid #16544)

• Depositing Lab: Bert Vogelstein
• Publication: Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96(25):14517-22.

Backbone

• Vector backbone: pBI-MCS-EGFP
• Backbone size w/o insert (bp): 5210
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p53
• Species: H. sapiens (human)
• Insert Size (bp): 1700
• Mutation: R175H
• Entrez Gene: TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Tet-responsive mutant p53 expression construct.