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CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA
(Plasmid #165437)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165437 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Customized Vector
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2277
  • Mutation
    H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residues 60-113 with H83L, H86L, and Q108L mutations) does not bind to RhoA
  • Promoter CMV
  • Tag / Fusion Protein
    • ShadowY, Clover(T153M/F223R) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA was a gift from Hideji Murakoshi (Addgene plasmid # 165437 ; http://n2t.net/addgene:165437 ; RRID:Addgene_165437)

  • For your References section:

    Photoactivatable CaMKII induces synaptic plasticity in single synapses. Shibata ACE, Ueda HH, Eto K, Onda M, Sato A, Ohba T, Nabekura J, Murakoshi H. Nat Commun. 2021 Feb 2;12(1):751. doi: 10.1038/s41467-021-21025-6. 10.1038/s41467-021-21025-6 PubMed 33531495
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