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PurposeExpresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 165431 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCustomized Vector
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametdTomato-P2A-paCaMKII
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SpeciesR. norvegicus (rat), Synthetic
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Insert Size (bp)3282
- Promoter CMV
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Tag
/ Fusion Protein
- tdTomato (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-tdTomato-P2A-paCaMKII was a gift from Hideji Murakoshi (Addgene plasmid # 165431 ; http://n2t.net/addgene:165431 ; RRID:Addgene_165431) -
For your References section:
Photoactivatable CaMKII induces synaptic plasticity in single synapses. Shibata ACE, Ueda HH, Eto K, Onda M, Sato A, Ohba T, Nabekura J, Murakoshi H. Nat Commun. 2021 Feb 2;12(1):751. doi: 10.1038/s41467-021-21025-6. 10.1038/s41467-021-21025-6 PubMed 33531495
Map uploaded by the depositor.
