-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 16543 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBI-MCS-EGFP
- Backbone size w/o insert (bp) 5210
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namep53
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1700
-
Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Tet-responsive p53 expression construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBI-p53 wt/EGFP was a gift from Bert Vogelstein (Addgene plasmid # 16543 ; http://n2t.net/addgene:16543 ; RRID:Addgene_16543) -
For your References section:
Identification and classification of p53-regulated genes. Yu J, Zhang L, Hwang PM, Rago C, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1999 Dec 7. 96(25):14517-22. 10.1073/pnas.96.25.14517 PubMed 10588737