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Addgene

Level 1 P2 OsUbiP:Cas9:NosT
(Plasmid #165424)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165424 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pICH47742
  • Backbone manufacturer
    Icon Genetics
  • Backbone size w/o insert (bp) 4352
  • Total vector size (bp) 10784
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Os Ubiquitin promoter :: Cas9 :: NosTerminator
  • Alt name
    RUbiP::Cas9::NosT
  • Species
    Synthetic
  • Insert Size (bp)
    6432
  • Promoter Os Ubiquitin promoter :: Cas9 :: NosTerminator

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Golden Gate BsaI (destroyed during cloning)
  • 3′ cloning site Golden Gate BsaI (destroyed during cloning)
  • 5′ sequencing primer LB Forward 5’-GTGGTGTAAACAAATTGACGC-3’
  • 3′ sequencing primer RB Reverse 5'-CTGGTGGCAGGATATATTGTGGTG-3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Level 1 P2 OsUbiP:Cas9:NosT was a gift from Wendy Harwood & John Innes Centre - Wheat Transformation and Genome Editing (Addgene plasmid # 165424 ; http://n2t.net/addgene:165424 ; RRID:Addgene_165424)

  • For your References section:

    CRISPR-Cas9 Based Genome Editing in Wheat. Smedley MA, Hayta S, Clarke M, Harwood WA. Curr Protoc. 2021 Mar;1(3):e65. doi: 10.1002/cpz1.65. 10.1002/cpz1.65 PubMed 33687760
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