Level 1 P1 OsActinP:Hygint:NosT
(Plasmid #165423)

• Purpose: GoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassette
• Depositing Labs: Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
• Publication: Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.1002/cpz1.65.

Backbone

• Vector backbone: pICH47732
• Backbone manufacturer: Icon Genetics
• Backbone size w/o insert (bp): 4352
• Total vector size (bp): 7273
• Vector type: Synthetic Biology ; L1 P1(R) Golden Gate (MoClo) hygromycin selection cassette used for wheat transformation

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
• Species: Synthetic
• Promoter: Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Golden Gate BsaI (not destroyed)
• 3′ cloning site: Golden Gate BsaI (not destroyed)
• 5′ sequencing primer: LB Forward 5’-GTGGTGTAAACAAATTGACGC-3’
• 3′ sequencing primer: RB Reverse 5'-CTGGTGGCAGGATATATTGTGGTG-3'

Resource Information

• Supplemental Documents:
  • Level 1 P1 OsActinP Hygint NosT.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry