Level 1 P1 OsActinP:Hygint:NosT
(Plasmid
#165423)
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PurposeGoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassette
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 165423 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepICH47732
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Backbone manufacturerIcon Genetics
- Backbone size w/o insert (bp) 4352
- Total vector size (bp) 7273
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Vector typeSynthetic Biology ; L1 P1(R) Golden Gate (MoClo) hygromycin selection cassette used for wheat transformation
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOs Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
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SpeciesSynthetic
- Promoter Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Golden Gate BsaI (not destroyed)
- 3′ cloning site Golden Gate BsaI (not destroyed)
- 5′ sequencing primer LB Forward 5’-GTGGTGTAAACAAATTGACGC-3’
- 3′ sequencing primer RB Reverse 5'-CTGGTGGCAGGATATATTGTGGTG-3' (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Level 1 P1 OsActinP:Hygint:NosT was a gift from John Innes Centre - Crop Transformation and Genome Editing Group & Wendy Harwood (Addgene plasmid # 165423 ; http://n2t.net/addgene:165423 ; RRID:Addgene_165423) -
For your References section:
CRISPR-Cas9 Based Genome Editing in Wheat. Smedley MA, Hayta S, Clarke M, Harwood WA. Curr Protoc. 2021 Mar;1(3):e65. doi: 10.1002/cpz1.65. 10.1002/cpz1.65 PubMed 33687760