pGoldenGreenGate-M (pGGG-M)
(Plasmid #165422)

• Purpose: (Empty Backbone) A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).
• Depositing Labs: Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
• Publication: Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.1002/cpz1.65.

Backbone

• Vector backbone: pGoldenGreenGate (pGGG) (pGreenII based level 2 Golden Gate (MoClo) compatible vector with additional LB)
• Backbone size (bp): 2678
• Modifications to backbone: Additional LB sequence.
• Vector type: Synthetic Biology ; Level 2 Golden Gate (MoClo) dual/binary vector used for Agrobacterium transformation of plants
• Promoter: Contain GG cassette and MCS with LacZ for blue/white screening
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: LB Forward 5’-GTGGTGTAAACAAATTGACGC-3’
• 3′ sequencing primer: RB-R1 Reverse 5’-CCCGCCAATATATCCTGTCA-3’

Resource Information

• Supplemental Documents:
  • pGoldenGreenGate - M (pGGG-M).gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: This plasmid is based on pGreen and therefore requires the helper plasmid pSoup (Addgene ID # 165419) to replicate in Agrobacterium.

Hellens, R.P., Edwards, E.A., Leyland, N.R. et al. pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol 42, 819–832 (2000). https://doi.org/10.1023/A:1006496308160