pBXPHM3-PCFT_NB
(Plasmid #165415)

• Purpose: Expression in E.coli of N terminally MBP tagged anti-GgPCFT nano body
• Depositing Lab: Simon Newstead
• Publication: Parker et al Nature. 2021 May 26. pii: 10.1038/s41586-021-03579-z. doi: 10.1038/s41586-021-03579-z.

Backbone

• Vector backbone: pBXNPHM3
• Backbone size w/o insert (bp): 6994
• Total vector size (bp): 7300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GgPCFT_NB
• Species: G. gallus (chicken); Llama
• Insert Size (bp): 381
• Entrez Gene: SLC46A1 (a.k.a. PCFT)
• Promoter: arabinose
• Tag / Fusion Protein:
  • His10-MBP-3C protease site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspQI (unknown if destroyed)
• 3′ cloning site: BspQI (unknown if destroyed)
• 5′ sequencing primer: MalE

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry