pBXPHM3-PCFT_NB
(Plasmid
#165415)
-
PurposeExpression in E.coli of N terminally MBP tagged anti-GgPCFT nano body
-
Depositing Lab
-
Sequence Information
-
Sequences (2) — Accept Affinity Reagent Sequence Policy
-
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 165415 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepBXNPHM3
- Backbone size w/o insert (bp) 6994
- Total vector size (bp) 7300
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameGgPCFT_NB
-
SpeciesG. gallus (chicken); Llama
-
Insert Size (bp)381
-
Entrez GeneSLC46A1 (a.k.a. PCFT)
- Promoter arabinose
-
Tag
/ Fusion Protein
- His10-MBP-3C protease site (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BspQI (unknown if destroyed)
- 3′ cloning site BspQI (unknown if destroyed)
- 5′ sequencing primer MalE (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBXPHM3-PCFT_NB was a gift from Simon Newstead (Addgene plasmid # 165415 ; http://n2t.net/addgene:165415 ; RRID:Addgene_165415) -
For your References section:
Structural basis of antifolate recognition and transport by PCFT. Parker JL, Deme JC, Kuteyi G, Wu Z, Huo J, Goldman ID, Owens RJ, Biggin PC, Lea SM, Newstead S. Nature. 2021 May 26. pii: 10.1038/s41586-021-03579-z. doi: 10.1038/s41586-021-03579-z. 10.1038/s41586-021-03579-z PubMed 34040256
