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Addgene

pCS2+ GCamp6-3NLS
(Plasmid #165401)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165401 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4105
  • Total vector size (bp) 5469
  • Vector type
    Mammalian Expression ; Avian, Xenopus, Zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nuclear Localization Signal
  • Alt name
    NLS
  • Insert Size (bp)
    1364
  • GenBank ID
  • Promoter SP6
  • Tag / Fusion Protein
    • GCamp6 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (destroyed during cloning)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ GCamp6-3NLS was a gift from Philipp Niethammer (Addgene plasmid # 165401 ; http://n2t.net/addgene:165401 ; RRID:Addgene_165401)

  • For your References section:

    The Cell Nucleus Serves as a Mechanotransducer of Tissue Damage-Induced Inflammation. Enyedi B, Jelcic M, Niethammer P. Cell. 2016 May 19;165(5):1160-1170. doi: 10.1016/j.cell.2016.04.016. 10.1016/j.cell.2016.04.016 PubMed 27203112
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