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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16540 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBJ1 neo
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Backbone manufacturerMark M Davis
- Backbone size w/o insert (bp) 6600
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFCER1G
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Alt namehuman FcR gamma chain
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SpeciesH. sapiens (human)
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Insert Size (bp)421
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GenBank IDNM_004106
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Entrez GeneFCER1G (a.k.a. FCRG)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (destroyed during cloning)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This construct contains only the coding sequence. It was first used in Lin S, Cicala C, Scharenberg AM, Kinet J-P (1996) Cell 85, 985-995. It can be sequenced with primers within the insert.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBJ1 neo-hu FceRI gamma was a gift from Jean Kinet (Addgene plasmid # 16540 ; http://n2t.net/addgene:16540 ; RRID:Addgene_16540) -
For your References section:
Characterization and expression of the gene for the human Fc receptor gamma subunit. Definition of a new gene family. Kuster H, Thompson H, Kinet JP. J Biol Chem 1990 Apr 15;265(11):6448-52. PubMed 2138619