Skip to main content
Addgene

p4xmTRE2-Luc
(Plasmid #16538)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16538 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBV-Luc
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size w/o insert (bp) 4867
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4xmTRE2
  • Alt name
    Mutant Tcf-4-binding sites
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains 4 copies of putative Tcf-4-binding sites TRE1. The following oligonucleotide pairs were used for dimerization to construct corresponding reporters in pBV-Luc: 5’-CTAGCTTGGC TTTCGCCTGA TTGGCTTTCG CCTGAG-3’ and 5’-CTAGCTCAGG CGAAAGCCAA TCAGGCGAAA GCCAAG-3’ for p4XmTRE2-Luc.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p4xmTRE2-Luc was a gift from Bert Vogelstein (Addgene plasmid # 16538 ; http://n2t.net/addgene:16538 ; RRID:Addgene_16538)

  • For your References section:

    PPARdelta is an APC-regulated target of nonsteroidal anti-inflammatory drugs. He TC, Chan TA, Vogelstein B, Kinzler KW. Cell. 1999 Oct 29. 99(3):335-45. 10.1016/S0092-8674(00)81664-5 PubMed 10555149
Related items:
Commonly requested with: