mNP-Luc (PPARdelta promoter)
(Plasmid #16532)

• Depositing Lab: Bert Vogelstein
• Publication: He et al Cell. 1999 Oct 29. 99(3):335-45.

Backbone

• Vector backbone: pBV-Luc
• Backbone manufacturer: Vogelstein Lab
• Backbone size w/o insert (bp): 4867
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPAR delta promoter NP
• Alt name: PPAR delta
• Species: H. sapiens (human)
• Insert Size (bp): 800
• Mutation: Mutant binding sites
• Entrez Gene: PPARD (a.k.a. FAAR, NR1C2, NUC1, NUCI, NUCII, PPARB)
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: PvuII (unknown if destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mNP represents fragment NP with both potential Tcf-4-binding sites mutated. TRE1 and TRE2 contain four repeats of the two Tcf-4-binding sites, respectively. mTRE1 and mTRE2 are mutant forms of TRE1 and TRE2.