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Addgene

GST-FAST-FL
(Plasmid #16523)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16523 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2TK
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FAST-1
  • Alt name
    Fast1
  • Alt name
    forkhead activin signal transducer-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1100
  • Entrez Gene
    FOXH1 (a.k.a. FAST-1, FAST1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To produce a GST-fusion protein (FAST-FL) containing the full-length hFAST-1, the entire ORF of hFAST-1 was PCR-amplified and cloned into the BamHI site of pGEX2TK (Pharmacia).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-FAST-FL was a gift from Bert Vogelstein (Addgene plasmid # 16523 ; http://n2t.net/addgene:16523 ; RRID:Addgene_16523)

  • For your References section:

    Characterization of human FAST-1, a TGF beta and activin signal transducer. Zhou S, Zawel L, Lengauer C, Kinzler KW, Vogelstein B. Mol Cell. 1998 Jul . 2(1):121-7. 10.1016/S1097-2765(00)80120-3 PubMed 9702198
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