CMV-Fast-1 H83R
(Plasmid
#16522)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16522 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCI-Neo
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFAST-1 H83R
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Alt nameFast1
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Alt nameforkhead activin signal transducer-1
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SpeciesH. sapiens (human)
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Insert Size (bp)1100
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MutationH83R mutant
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Entrez GeneFOXH1 (a.k.a. FAST-1, FAST1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-Fast-1 H83R was a gift from Bert Vogelstein (Addgene plasmid # 16522 ; http://n2t.net/addgene:16522 ; RRID:Addgene_16522) -
For your References section:
Characterization of human FAST-1, a TGF beta and activin signal transducer. Zhou S, Zawel L, Lengauer C, Kinzler KW, Vogelstein B. Mol Cell. 1998 Jul . 2(1):121-7. 10.1016/S1097-2765(00)80120-3 PubMed 9702198
Map uploaded by the depositor.
