Skip to main content
Addgene

pCI-neo beta catenin delta45
(Plasmid #16520)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16520 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta catenin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2350
  • Mutation
    Delta 45 (HCT116 mutation)
  • Entrez Gene
    CTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HCT116 has a 3-bp deletion that removes one amino acid (Ser45).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-neo beta catenin delta45 was a gift from Bert Vogelstein (Addgene plasmid # 16520 ; http://n2t.net/addgene:16520 ; RRID:Addgene_16520)

  • For your References section:

    Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC. Morin PJ, Sparks AB, Korinek V, Barker N, Clevers H, Vogelstein B, Kinzler KW. Science. 1997 Mar 21. 275(5307):1787-90. 10.1126/science.275.5307.1787 PubMed 9065402
Related items:
Commonly requested with: