pCI-neo beta catenin S33Y
(Plasmid #16519)

• Depositing Lab: Bert Vogelstein
• Publication: Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Backbone

• Vector backbone: pCI-neo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta catenin
• Species: H. sapiens (human)
• Insert Size (bp): 2350
• Mutation: S33Y (SW48 mutation)
• Entrez Gene: CTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SW48 has a C-to-A missense mutation that changes Ser33 to Tyr.

Note that the NheI site is not intact and cannot be used for sub-cloning.