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Addgene

pBR322 14-3-3 Promoter
(Plasmid #16517)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16517 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 4361
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    14-3-3 promoter
  • Alt name
    14-3-3 sigma
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    9500
  • GenBank ID
    AF029081
  • Entrez Gene
    SFN (a.k.a. YWHAS)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer pBRrevBam
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBR322 14-3-3 Promoter was a gift from Bert Vogelstein (Addgene plasmid # 16517 ; http://n2t.net/addgene:16517 ; RRID:Addgene_16517)

  • For your References section:

    14-3-3 sigma is a p53-regulated inhibitor of G2/M progression. Hermeking H, Lengauer C, Polyak K, He TC, Zhang L, Thiagalingam S, Kinzler KW, Vogelstein B. Mol Cell. 1997 Dec . 1(1):3-11. 10.1016/S1097-2765(00)80002-7 PubMed 9659898
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