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Addgene

PROM5_MprbcL_short_chlo
(Plasmid #165161)

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Item Catalog # Description Quantity Price (USD)
Plasmid 165161 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pUAP4
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Marchantia polymorpha rbcL promoter region

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PROM5_MprbcL_short_chlo was a gift from Jim Haseloff (Addgene plasmid # 165161 ; http://n2t.net/addgene:165161 ; RRID:Addgene_165161)

  • For your References section:

    Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts. Frangedakis E, Guzman-Chavez F, Rebmann M, Markel K, Yu Y, Perraki A, Tse SW, Liu Y, Rever J, Sauret-Gueto S, Goffinet B, Schneider H, Haseloff J. ACS Synth Biol. 2021 Jul 16;10(7):1651-1666. doi: 10.1021/acssynbio.0c00637. Epub 2021 Jun 7. 10.1021/acssynbio.0c00637 PubMed 34097383
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