BDS-2 3x MUT (p53 binding site)
(Plasmid #16516)

• Depositing Lab: Bert Vogelstein
• Publication: Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

Backbone

• Vector backbone: pBV-Luc
• Backbone manufacturer: Vogelstein Lab
• Backbone size w/o insert (bp): 4900
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p53 binding site
• Alt name: 14-3-3 sigma promoter
• Species: H. sapiens (human)
• Mutation: Mutant p53 binding sites
• Entrez Gene: SFN (a.k.a. YWHAS)
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This reporter contains 3 copies of the mutant p53-binding site from the 14-3-3 sigma promoter. The following oligonucleotide pairs were used: 5'-CCTGTAGAAT TATCCCAGAA ATTTCCCTAC TCCGTAC-3' and 5'-GGAGTAGGGA AATTTCTGGG ATAATTCTAC AGGGTAC-3' for BDS-2*, altered at critical p53-binding residues.