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pLPC-mCh-ACTB-P2A-EGFP
(Plasmid #165158)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165158 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLPC
  • Backbone manufacturer
    Scott Lowe
  • Backbone size w/o insert (bp) 5795
  • Total vector size (bp) 7439
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-ACTB-P2A-EGFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    732
  • Entrez Gene
    ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mCherry (N terminal on insert)
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAGGCGTGTACGGTGGGA
  • 3′ sequencing primer CTTGCCAAACCTACAGGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC-mCh-ACTB-P2A-EGFP was a gift from Taekjip Ha (Addgene plasmid # 165158 ; http://n2t.net/addgene:165158 ; RRID:Addgene_165158)

  • For your References section:

    Cas9 deactivation with photocleavable guide RNAs. Zou RS, Liu Y, Wu B, Ha T. Mol Cell. 2021 Apr 1;81(7):1553-1565.e8. doi: 10.1016/j.molcel.2021.02.007. Epub 2021 Mar 3. 10.1016/j.molcel.2021.02.007 PubMed 33662274
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