pSprayerHE
(Plasmid
#165150)
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PurposeSprayer plasmid for PROBER encoding BASU-GAL4 fusion (High Expression)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 165150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC-like
- Total vector size (bp) 4537
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBASU-GAL4
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSprayerHE was a gift from Paul Khavari (Addgene plasmid # 165150 ; http://n2t.net/addgene:165150 ; RRID:Addgene_165150) -
For your References section:
PROBER identifies proteins associated with programmable sequence-specific DNA in living cells. Mondal S, Ramanathan M, Miao W, Meyers RM, Rao D, Lopez-Pajares V, Siprashvili Z, Reynolds DL, Porter DF, Ferguson I, Neela P, Zhao Y, Meservey LM, Guo M, Yang YY, Li L, Wang Y, Khavari PA. Nat Methods. 2022 Aug;19(8):959-968. doi: 10.1038/s41592-022-01552-w. Epub 2022 Aug 4. 10.1038/s41592-022-01552-w PubMed 35927480