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PurposeExpresses mRuby4(1-10) in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 165143 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepcDNA3.1(+)
- Total vector size (bp) 6035
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemRuby4(1-10)
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SpeciesSynthetic
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Insert Size (bp)636
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-mRuby4(1-10) was a gift from Daichi Kamiyama (Addgene plasmid # 165143 ; http://n2t.net/addgene:165143 ; RRID:Addgene_165143) -
For your References section:
Multiplexed labeling of cellular proteins with split fluorescent protein tags. Tamura R, Jiang F, Xie J, Kamiyama D. Commun Biol. 2021 Feb 26;4(1):257. doi: 10.1038/s42003-021-01780-4. 10.1038/s42003-021-01780-4 PubMed 33637968
Map uploaded by the depositor.
