pHR TCF4
(Plasmid #16514)

• Depositing Lab: Bert Vogelstein
• Publication: Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

Backbone

• Vector backbone: pHR
• Backbone size w/o insert (bp): 9200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TCF4
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• Entrez Gene: TCF7L2 (a.k.a. TCF-4, TCF4)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See map (not destroyed)
• 3′ cloning site: See map (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The TCF4 in this construct has a Q27R mutation. It is not known to affect the function.

According to the depositing scientist, other mutations may be present but they have no functional consequences on standard assays for Tcf4 activity.