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pGEX4T1-ISG15 C78SN13Y
(Plasmid #165106)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165106 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T-1
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ISG15
  • Alt name
    UCRP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    471
  • Mutation
    C78SN13Y; The sequence of ISG15-C78S/N13Y contains a cysteine to serine mutation at residue 78 which has been found to increase stability of the protein during purification, concentration, and storage. It also contains an asparagine to tyrosine mutation at residue 13 to produce a solvent accessible site for labeling with 125Iodine
  • Entrez Gene
    ISG15 (a.k.a. G1P2, IFI15, IMD38, IP17, UCRP, hUCRP)
  • Tag / Fusion Protein
    • GST from plasmid. (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T1-ISG15 C78SN13Y was a gift from Arthur Haas (Addgene plasmid # 165106 ; http://n2t.net/addgene:165106 ; RRID:Addgene_165106)

  • For your References section:

    Crystal structure of the interferon-induced ubiquitin-like protein ISG15. Narasimhan J, Wang M, Fu Z, Klein JM, Haas AL, Kim JJ. J Biol Chem. 2005 Jul 22. 280(29):27356-65. 10.1074/jbc.M502814200 PubMed 15917233
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