pGEX4T-1-6AlanineHE1
(Plasmid #165098)

• Purpose: bacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1
• Depositing Lab: Arthur Haas
• Publication: Haas Lab plasmids (unpublished)

Backbone

• Vector backbone: pGEX4T1
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 4900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Uba1
• Alt name: UBA1A
• Species: H. sapiens (human)
• Insert Size (bp): 3195
• Mutation: None
• Entrez Gene: UBA1 (a.k.a. A1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X, VEXAS)
• Tag / Fusion Protein:
  • GST-6-alanine (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C.