pET19b-EcMscL-TEV-mEGFP
(Plasmid #165097)

• Purpose: Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.
• Depositing Lab: Neha Kamat
• Publication: Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. pii: 1814775116. doi: 10.1073/pnas.1814775116.

Backbone

• Vector backbone: pET19b
• Backbone size w/o insert (bp): 5600
• Total vector size (bp): 6800
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: E. Coli Mechanosensitive Channel of Large Conductance
• Alt name: MscLGFP
• Alt name: EcMscL-mEGFP
• Species: E. coli
• Insert Size (bp): 1236
• Entrez Gene: mscL (a.k.a. b3291, ECK3277, yhdC)
• Promoter: T7
• Tag / Fusion Protein:
  • monomeric Enhanced Green Fluorescent Protein (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 fwd
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • pET19b-EcMscL-TEV-meGFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry