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Addgene

pET32a-Spike-S-4P
(Plasmid #165094)

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Item Catalog # Description Quantity Price (USD)
Plasmid 165094 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET-32a(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166
  • Species
    SARS-CoV2
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET32a-Spike-S-4P was a gift from Rick Tarleton (Addgene plasmid # 165094 ; http://n2t.net/addgene:165094 ; RRID:Addgene_165094)

  • For your References section:

    A flexible, pan-species, multi-antigen platform for the detection and monitoring of SARS-CoV-2-specific antibody responses. Shen H, Forgacs D, Chapla D, Moremen KW, Wells L, Hamer SA, Tompkins SM, Ross TM, Rouphael N, Edupuganti S, Collins MH, Tarleton RL. medRxiv [Preprint]. 2021 Jan 27:2021.01.20.21249279. doi: 10.1101/2021.01.20.21249279. 10.1101/2021.01.20.21249279 PubMed 33532799
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