pMasterK
(Plasmid #165081)

• Purpose: Episomal expression of reprogramming factors.
• Depositing Lab: Hideyuki Okano
• Publication: Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi: 10.1016/j.scr.2021.102380. Epub 2021 Apr 30.

Backbone

• Vector backbone: pMaster
• Vector type: Mammalian Expression ; Episomal
• Selectable markers: Neomycin (select with G418), Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Plasmid is unstable and prone to recombination. Test 3-5 small colonies to ensure the full plasmid is intact.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: OCT4
• Alt name: POU5F1
• Species: H. sapiens (human)
• Insert Size (bp): 1080
• Entrez Gene: POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)

Gene/Insert 2

• Gene/Insert name: SOX2
• Species: H. sapiens (human)
• Insert Size (bp): 951
• Entrez Gene: SOX2 (a.k.a. ANOP3, MCOPS3)

Gene/Insert 3

• Gene/Insert name: KLF4
• Species: H. sapiens (human)
• Insert Size (bp): 1410
• Entrez Gene: KLF4 (a.k.a. EZF, GKLF)

Gene/Insert 4

• Gene/Insert name: MYC
• Alt name: C-MYC
• Species: H. sapiens (human)
• Insert Size (bp): 1320
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Gene/Insert 5

• Gene/Insert name: LRH1
• Species: H. sapiens (human)
• Insert Size (bp): 1488
• Entrez Gene: NR5A2 (a.k.a. B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2)

Gene/Insert 6

• Gene/Insert name: NANOG
• Species: H. sapiens (human)
• Insert Size (bp): 915
• Entrez Gene: NANOG

Gene/Insert 7

• Gene/Insert name: lin28
• Species: H. sapiens (human)
• Insert Size (bp): 627
• Entrez Gene: LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)

Gene/Insert 8

• Gene/Insert name: EGFP
• Species: Aequorea victoria
• Insert Size (bp): 710

Gene/Insert 9

• Gene/Insert name: HSV TK
• Species: Herpes Simplex Virus
• Insert Size (bp): 1131

Gene/Insert 10

• Gene/Insert name: KDM4D
• Species: Callithrix jacchus
• Insert Size (bp): 1569
• Entrez Gene: KDM4D (a.k.a. JMJD2D)

Gene/Insert 11

• Gene/Insert name: GLIS1
• Species: H. sapiens (human)
• Insert Size (bp): 1860
• Entrez Gene: GLIS1

Resource Information

• Supplemental Documents:
  • pMasterK.gb
• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's NGS found that the bGH poly(A) signal between one of the oris and Lin28 is absent as compared to the annotated sequence provided by the depositor.

Please note that this plasmid contains repetitive elements which are prone to recombination. Screen 3-5 small colonies to ensure the full plasmid is selected. For example, the restriction enzyme NheI can be used to check the plasmid size as shown in Addgene's Diagnostic Digest.