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Addgene

pMasterK
(Plasmid #165081)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165081 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMaster
  • Vector type
    Mammalian Expression ; Episomal
  • Selectable markers
    Neomycin (select with G418), Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Plasmid is unstable and prone to recombination. Test 3-5 small colonies to ensure the full plasmid is intact.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    OCT4
  • Alt name
    POU5F1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1080
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)

Gene/Insert 2

  • Gene/Insert name
    SOX2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    951
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)

Gene/Insert 3

  • Gene/Insert name
    KLF4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1410
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)

Gene/Insert 4

  • Gene/Insert name
    MYC
  • Alt name
    C-MYC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1320
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Gene/Insert 5

  • Gene/Insert name
    LRH1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1488
  • Entrez Gene
    NR5A2 (a.k.a. B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2)

Gene/Insert 6

  • Gene/Insert name
    NANOG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    915
  • Entrez Gene
    NANOG

Gene/Insert 7

  • Gene/Insert name
    lin28
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    627
  • Entrez Gene
    LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)

Gene/Insert 8

  • Gene/Insert name
    EGFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    710

Gene/Insert 9

  • Gene/Insert name
    HSV TK
  • Species
    Herpes Simplex Virus
  • Insert Size (bp)
    1131

Gene/Insert 10

  • Gene/Insert name
    KDM4D
  • Species
    Callithrix jacchus
  • Insert Size (bp)
    1569
  • Entrez Gene
    KDM4D (a.k.a. JMJD2D)

Gene/Insert 11

  • Gene/Insert name
    GLIS1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1860
  • Entrez Gene
    GLIS1

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid contains repetitive elements which are prone to recombination. Screen 3-5 small colonies to ensure the full plasmid is selected. For example, the restriction enzyme NheI can be used to check the plasmid size as shown in Addgene's Diagnostic Digest.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMasterK was a gift from Hideyuki Okano (Addgene plasmid # 165081)

  • For your References section:

    Establishing an induced pluripotent stem cell line from neonatal common marmoset fibroblasts by an all-in-one episomal vector approach. Yoshimatsu S, Qian E, Sato T, Yamamoto M, Ishikawa M, Okano H. Stem Cell Res. 2021 May;53:102380. doi: 10.1016/j.scr.2021.102380. Epub 2021 Apr 30. 10.1016/j.scr.2021.102380 PubMed 34088009
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