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pPBCAG-rtTAM2-2A-SOX17-GR-IH
(Plasmid #165079)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165079 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPBCAG
  • Vector type
    Mammalian Expression ; PiggyBac transposition
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    rtTA-M2
  • Alt name
    rtTAM2
  • Species
    Synthetic
  • Insert Size (bp)
    741

Gene/Insert 2

  • Gene/Insert name
    SOX17
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1242
  • GenBank ID
    NM_022454.4
  • Entrez Gene
    SOX17 (a.k.a. VUR3)
  • Tag / Fusion Protein
    • Glucocorticoid Receptor (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPBCAG-rtTAM2-2A-SOX17-GR-IH was a gift from Hideyuki Okano (Addgene plasmid # 165079 ; http://n2t.net/addgene:165079 ; RRID:Addgene_165079)

  • For your References section:

    Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts of Multiple Mammalian Species. Yoshimatsu S, Nakajima M, Iguchi A, Sanosaka T, Sato T, Nakamura M, Nakajima R, Arai E, Ishikawa M, Imaizumi K, Watanabe H, Okahara J, Noce T, Takeda Y, Sasaki E, Behr R, Edamura K, Shiozawa S, Okano H. Stem Cell Reports. 2021 Mar 23. pii: S2213-6711(21)00132-6. doi: 10.1016/j.stemcr.2021.03.002. 10.1016/j.stemcr.2021.03.002 PubMed 33798453
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