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Addgene

pCMV-Neo-Bam APC
(Plasmid #16507)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16507 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Neo-Bam
  • Backbone manufacturer
    Vogelstein lab (Addgene plasmid# 16440)
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    APC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    9000
  • Entrez Gene
    APC (a.k.a. BTPS2, DESMD, DP2, DP2.5, DP3, GS, PPP1R46)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer Bglob-intron-F
  • 3′ sequencing primer Bglob-pA-R (5'-ttttggcagagggaaaaaga-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Neo-Bam APC was a gift from Bert Vogelstein (Addgene plasmid # 16507 ; http://n2t.net/addgene:16507 ; RRID:Addgene_16507)

  • For your References section:

    Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC. Morin PJ, Sparks AB, Korinek V, Barker N, Clevers H, Vogelstein B, Kinzler KW. Science. 1997 Mar 21. 275(5307):1787-90. 10.1126/science.275.5307.1787 PubMed 9065402
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