pJT11F
(Plasmid #165062)

• Purpose: Introducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiae
• Depositing Lab: Claudia Vickers
• Publication: Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi: 10.1038/s41467-021-21313-1.

Backbone

• Vector backbone: pRS425
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: T-RPL3>ERG20-N127W>P-GAL1-P-GAL10>Y-FAST>T-ALD6-P-GAL2>ClLS>T-RPL41B
• Species: Other
• Mutation: WT

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pJT11F.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry