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Addgene

pJAID5VY
(Plasmid #165048)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165048 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
  • Species
    Other
  • Mutation
    OsTIR1 is codon-optimised for expression in S. cerevisiae

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJAID5VY was a gift from Claudia Vickers (Addgene plasmid # 165048 ; http://n2t.net/addgene:165048 ; RRID:Addgene_165048)

  • For your References section:

    Auxin-mediated protein depletion for metabolic engineering in terpene-producing yeast. Lu Z, Peng B, Ebert BE, Dumsday G, Vickers CE. Nat Commun. 2021 Feb 16;12(1):1051. doi: 10.1038/s41467-021-21313-1. 10.1038/s41467-021-21313-1 PubMed 33594068
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