pJAID6A
(Plasmid
#165046)
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PurposeIntegrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 165046 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameP-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
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SpeciesOther
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MutationWT
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJAID6A was a gift from Claudia Vickers (Addgene plasmid # 165046 ; http://n2t.net/addgene:165046 ; RRID:Addgene_165046) -
For your References section:
Auxin-mediated protein depletion for metabolic engineering in terpene-producing yeast. Lu Z, Peng B, Ebert BE, Dumsday G, Vickers CE. Nat Commun. 2021 Feb 16;12(1):1051. doi: 10.1038/s41467-021-21313-1. 10.1038/s41467-021-21313-1 PubMed 33594068