RPL13A_PQR_mScarlet_NOL_LoxP_PQR_zeo_PQR_MCS_RPL13A-3'UTR
(Plasmid
#165043)
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PurposeRepair template for CAS9 complex genome editing
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 165043 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepuc19 without a promoter for the insert
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RPL13A_PQR_mScarlet_NOL_LoxP_PQR_zeo_PQR_MCS_RPL13A-3'UTR was a gift from Brian Chen (Addgene plasmid # 165043 ; http://n2t.net/addgene:165043 ; RRID:Addgene_165043) -
For your References section:
Generating stable cell lines with quantifiable protein production using CRISPR/Cas9-mediated knock-in. Lo CA, Greben AW, Chen BE. Biotechniques. 2017 Apr 1;62(4):165-174. doi: 10.2144/000114534. 10.2144/000114534 PubMed 28403807