pTXB1.Tat(48-57)-Cre recombinase
(Plasmid
#165042)
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PurposeExpression of Tat(48-57) conjugated Cre recombinase for protein purification (Intein-chitin binding domain fussion)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 165042 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTXB1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCre recombinase
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Speciesbacteriophage P1
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Tags
/ Fusion Proteins
- Tat(48-57) (N terminal on insert)
- Mxe Intein-Chitin binding domain (C terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains an A207T mutation in Cre. This mutation is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTXB1.Tat(48-57)-Cre recombinase was a gift from Dokyun Na (Addgene plasmid # 165042 ; http://n2t.net/addgene:165042 ; RRID:Addgene_165042) -
For your References section:
Identification of efficient prokaryotic cell-penetrating peptides with applications in bacterial biotechnology. Lee HM, Ren J, Tran KM, Jeon BM, Park WU, Kim H, Lee KE, Oh Y, Choi M, Kim DS, Na D. Commun Biol. 2021 Feb 15;4(1):205. doi: 10.1038/s42003-021-01726-w. 10.1038/s42003-021-01726-w PubMed 33589718