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Addgene

pET21a.Tat(48-57)-I-SceI
(Plasmid #165041)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165041 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    I-SceI
  • Species
    S. cerevisiae (budding yeast)
  • Tags / Fusion Proteins
    • Tat(48-57) (N terminal on insert)
    • 6XHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a.Tat(48-57)-I-SceI was a gift from Dokyun Na (Addgene plasmid # 165041 ; http://n2t.net/addgene:165041 ; RRID:Addgene_165041)

  • For your References section:

    Identification of efficient prokaryotic cell-penetrating peptides with applications in bacterial biotechnology. Lee HM, Ren J, Tran KM, Jeon BM, Park WU, Kim H, Lee KE, Oh Y, Choi M, Kim DS, Na D. Commun Biol. 2021 Feb 15;4(1):205. doi: 10.1038/s42003-021-01726-w. 10.1038/s42003-021-01726-w PubMed 33589718
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