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mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
(Plasmid #165029)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165029 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTriEX
  • Backbone size w/o insert (bp) 5646
  • Total vector size (bp) 6813
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
  • Species
    Synthetic
  • Insert Size (bp)
    1167
  • Promoter T7
  • Tag / Fusion Protein
    • mCherry

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer mCherry-for
  • 3′ sequencing primer atctcagtggtatttgtgagccag
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C) was a gift from Yubin Zhou (Addgene plasmid # 165029 ; http://n2t.net/addgene:165029 ; RRID:Addgene_165029)

  • For your References section:

    Expanding the Chemogenetic Toolbox by Circular Permutation. Lee YT, He L, Zhou Y. J Mol Biol. 2020 May 1;432(10):3127-3136. doi: 10.1016/j.jmb.2020.03.033. Epub 2020 Apr 8. 10.1016/j.jmb.2020.03.033 PubMed 32277990
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