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Addgene

pLJM1-Flag-Rheb-4Rs
(Plasmid #165022)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165022 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLJM1
  • Backbone manufacturer
    Available at Addgene (#19319)
  • Backbone size w/o insert (bp) 7400
  • Total vector size (bp) 7955
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rheb-4Rs
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    555
  • Mutation
    change lysine residues on position 109, 135, 151, 178 to arginines
  • GenBank ID
    NM_005614.4
  • Entrez Gene
    RHEB (a.k.a. RHEB2)
  • Tag / Fusion Protein
    • flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJM1-Flag-Rheb-4Rs was a gift from Ken Inoki (Addgene plasmid # 165022 ; http://n2t.net/addgene:165022 ; RRID:Addgene_165022)

  • For your References section:

    Amino Acids Enhance Polyubiquitination of Rheb and Its Binding to mTORC1 by Blocking Lysosomal ATXN3 Deubiquitinase Activity. Yao Y, Hong S, Ikeda T, Mori H, MacDougald OA, Nada S, Okada M, Inoki K. Mol Cell. 2020 Nov 5;80(3):437-451.e6. doi: 10.1016/j.molcel.2020.10.004. 10.1016/j.molcel.2020.10.004 PubMed 33157014
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