TTVTFLPTPSLILQT_epitope
(Plasmid
#165021)
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PurposeExpression of CMV UL44 (38-52) fused to CD74
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 165021 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLX301 (Addgene #25895)
- Backbone size w/o insert (bp) 9531
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCMV UL44 (38-52)
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Alt nameTTVTFLPTPSLILQT
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SpeciesHuman cytomegalovirus (CMV)
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Insert Size (bp)774
- Promoter CMV
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Tag
/ Fusion Protein
- CD74 at N-term; cathepsin S cleavage site (GRWHTVGL) and YM spacer between CD74 and epitope (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TTVTFLPTPSLILQT_epitope was a gift from Matthew Meyerson (Addgene plasmid # 165021 ; http://n2t.net/addgene:165021 ; RRID:Addgene_165021) -
For your References section:
Antigen identification for HLA class I- and HLA class II-restricted T cell receptors using cytokine-capturing antigen-presenting cells. Lee MN, Meyerson M. Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 10.1126/sciimmunol.abf4001. 10.1126/sciimmunol.abf4001 PubMed 33483338