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Addgene

AQTFAATHNPWASQA_epitope
(Plasmid #165019)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165019 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX301 (Addgene #25895)
  • Backbone size w/o insert (bp) 9531
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CMV UL86 (1222-1236)
  • Alt name
    AQTFAATHNPWASQA
  • Species
    Human cytomegalovirus (CMV)
  • Insert Size (bp)
    774
  • Promoter CMV
  • Tag / Fusion Protein
    • CD74 at N-term; cathepsin S cleavage site (GRWHTVGL) and YM spacer between CD74 and epitope (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AQTFAATHNPWASQA_epitope was a gift from Matthew Meyerson (Addgene plasmid # 165019 ; http://n2t.net/addgene:165019 ; RRID:Addgene_165019)

  • For your References section:

    Antigen identification for HLA class I- and HLA class II-restricted T cell receptors using cytokine-capturing antigen-presenting cells. Lee MN, Meyerson M. Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 10.1126/sciimmunol.abf4001. 10.1126/sciimmunol.abf4001 PubMed 33483338
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