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Addgene

pCRII Pig5
(Plasmid #16499)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16499 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRII
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3900
  • Vector type
    Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pig5
  • Alt name
    TMEM16J
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    850
  • Mutation
    Partial cDNA clone
  • Entrez Gene
    ANO9 (a.k.a. PIG5, TMEM16J, TP53I5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRII Pig5 was a gift from Bert Vogelstein (Addgene plasmid # 16499 ; http://n2t.net/addgene:16499 ; RRID:Addgene_16499)

  • For your References section:

    A model for p53-induced apoptosis. Polyak K, Xia Y, Zweier JL, Kinzler KW, Vogelstein B. Nature. 1997 Sep 18. 389(6648):300-5. 10.1038/38525 PubMed 9305847
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