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Addgene

pCAG-TWIN-STREP-FLAG-FLCN
(Plasmid #164981)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164981 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG TWIN STREP FLAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLCN
  • Alt name
    Folliculin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1740
  • Entrez Gene
    FLCN (a.k.a. BHD, DENND8B, FLCL)
  • Promoter CAG
  • Tag / Fusion Protein
    • 2xSTREP FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pCAG-F: CTTCTTCTTTTTCCTACAGCTCCTGGGC
  • 3′ sequencing primer pCAG-R: CAGTGGTATTTGTGAGCCAGGGCATT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-TWIN-STREP-FLAG-FLCN was a gift from Roberto Zoncu (Addgene plasmid # 164981 ; http://n2t.net/addgene:164981 ; RRID:Addgene_164981)

  • For your References section:

    Structural mechanism of a Rag GTPase activation checkpoint by the lysosomal folliculin complex. Lawrence RE, Fromm SA, Fu Y, Yokom AL, Kim DJ, Thelen AM, Young LN, Lim CY, Samelson AJ, Hurley JH, Zoncu R. Science. 2019 Nov 22;366(6468):971-977. doi: 10.1126/science.aax0364. Epub 2019 Oct 31. 10.1126/science.aax0364 PubMed 31672913
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