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Addgene

POLArISact
(Plasmid #164971)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164971 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified pcDNA3
  • Backbone manufacturer
    Zhao et. al. DOI: 10.1126/science.1208592
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    POLArISact
  • Alt name
    cpGFP-Adhiron (connected with an alpha helical linker)
  • Species
    Synthetic
  • Insert Size (bp)
    1074
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (additional HindIII site in insert) (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.06.20.116178v2 full for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    POLArISact was a gift from Sumio Terada (Addgene plasmid # 164971 ; http://n2t.net/addgene:164971 ; RRID:Addgene_164971)

  • For your References section:

    POLArIS, a versatile probe for molecular orientation, revealed actin filaments associated with microtubule asters in early embryos. Sugizaki A, Sato K, Chiba K, Saito K, Kawagishi M, Tomabechi Y, Mehta SB, Ishii H, Sakai N, Shirouzu M, Tani T, Terada S. Proc Natl Acad Sci U S A. 2021 Mar 16;118(11). pii: 2019071118. doi: 10.1073/pnas.2019071118. 10.1073/pnas.2019071118 PubMed 33674463
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