pDestTol2-actinb-kcna1a-IRES-EGFP
(Plasmid #164965)

• Purpose: Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP
• Depositing Lab: GuangJun Zhang
• Publication: Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi: 10.1534/genetics.120.303390. Epub 2020 Jun 16.

Backbone

• Vector backbone: pDestTol2pA2
• Backbone size w/o insert (bp): 4179
• Total vector size (bp): 12696
• Vector type: Tol2 transposon destination vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: kcna1a
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1479
• Entrez Gene: kcna1a (a.k.a. Kv1., kcn, kcna1, si:ch73-333m22.1, wu:fa27a, wu:fa27a01)
• Promoter: actinb (actb2) promoter
• Tag / Fusion Protein:
  • IRES-EGFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13R
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The DNA sequence was amplified from pooled wild type TAB zebrafish embryos. There is a likely polymorphism, K278M, which is not listed in the current ENSEMBL database. This is possibly due to incomplete zebrafish genome annotation, GRCz11.