pDONR221-kcnt1a
(Plasmid
#164961)
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PurposeZebrafish kcnt1a gene CDS
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164961 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2550
- Total vector size (bp) 5973
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Vector typeGateway entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namekcnt1a
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Alt namekcnt1
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)3423
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Entrez Genekcnt1
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221-kcnt1a was a gift from GuangJun Zhang (Addgene plasmid # 164961 ; http://n2t.net/addgene:164961 ; RRID:Addgene_164961) -
For your References section:
Phylogenetic and developmental analyses indicate complex functions of Calcium-Activated Potassium Channels in zebrafish embryonic development. Silic MR, Black MM, Zhang G. Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.329. 10.1002/dvdy.329 PubMed 33728688