pENTR-D-TOPO-kcnmb3
(Plasmid
#164955)
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PurposeZebrafish kcnmb3 gene CDS
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164955 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR-D-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2580
- Total vector size (bp) 3453
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Vector typePCR cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namekcnmb3
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Alt namesi:ch211-38m6.7
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)873
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Entrez Genesi:ch211-38m6.7
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-D-TOPO-kcnmb3 was a gift from GuangJun Zhang (Addgene plasmid # 164955 ; http://n2t.net/addgene:164955 ; RRID:Addgene_164955) -
For your References section:
Phylogenetic and developmental analyses indicate complex functions of Calcium-Activated Potassium Channels in zebrafish embryonic development. Silic MR, Black MM, Zhang G. Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.329. 10.1002/dvdy.329 PubMed 33728688