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pDestTol2-pax3a-kcnj13-IRES-EGFP
(Plasmid #164950)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164950 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDestTol2pA2
  • Backbone size w/o insert (bp) 4179
  • Total vector size (bp) 12556
  • Vector type
    Tol2 transposon destination vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kcnj13
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1096
  • Entrez Gene
    kcnj13 (a.k.a. ja, kir7, kir7.1, ob)
  • Promoter pax3a promoter -5.4k
  • Tag / Fusion Protein
    • IRES-EGFP (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDestTol2-pax3a-kcnj13-IRES-EGFP was a gift from GuangJun Zhang (Addgene plasmid # 164950 ; http://n2t.net/addgene:164950 ; RRID:Addgene_164950)

  • For your References section:

    Potassium Channel-Associated Bioelectricity of the Dermomyotome Determines Fin Patterning in Zebrafish. Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, Mittal SK, Zhang G. Genetics. 2020 Aug;215(4):1067-1084. doi: 10.1534/genetics.120.303390. Epub 2020 Jun 16. 10.1534/genetics.120.303390 PubMed 32546498
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